Molecular Cloning of Hemoglobin Alpha-chain Gene from Pantholops hodgsonii, a Hypoxic Tolerance Species
2007
Yingzhong, Yang (Qinghai University Medical School, Xining, P. R. China) | Droma, Yunden (Shinshu University School of Medicine, Matsumoto, Japan) | Guoen, Jin (Qinghai University Medical School, Xining, P. R. China) | Zhenzhong, Bai (Qinghai University Medical School, Xining, P. R. China) | Lan, Ma (Qinghai University Medical School, Xining, P. R. China) | Haixia, Yun (Qinghai University Medical School, Xining, P. R. China) | Yue, Cao (Qinghai University Medical School, Xining, P. R. China) | Kubo, Keishi (Shinshu University School of Medicine, Matsumoto, Japan) | Rili, Ge (Qinghai University Medical School, Xining, P. R. China), E-mail: [email protected]
To investigate the possible mechanisms of high-altitude native animals in adapting to high altitude, we cloned hemoglobin alpha-chain (alpha-chain Hb) gene from Pantholops hodgsonii, an animal species that indigenously lives at elevations of 3700-5500 m on the Qinghai-Tibetan plateau. Using reverse transcription polymerase chain reaction (RT-PCR) technique, the alpha-chain Hb gene was amplified from total RNA in the liver of the Pantholops hodgsonii. TA cloning technique was used and the PCR product was cloned into pGEM-T vector.
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