Overexpressed Drosophila DNA Methyltransferase 2 Isoform C Interacts with Hsp70 in Vivo
2007
Roder, Karim (Institute of Molecular Biology, Academia Sinica, Taiwan, Republic of China), E-mail: [email protected]
Shen and colleagues (Lin et al., 2004) have recently shown that overexpression of the Drosophila DNA methyltransferase 2 isoform C, dDnmt2c, extended life span of fruit flies, probably due to increased expression of small heat shock proteins such as Hsp22 or Hsp26. Here, I demonstrate with immunoprecipitations that overexpressed dDnmt2c interacts with endogenous Hsp70 protein in vivo in S2 cells. However, its C-terminal half, dDnmt2c(178-345) forms approximately 10-fold more Hsp70-containing protein complexe than wild-type dDnmt2c.
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