Cloning of Phospholipase D from Grape Berry and Its Expression under Heat Acclimation
2007
Wan, Si-Bao (China Agricultural University, Beijing, China) | Wang, Wei (China Agricultural University, Beijing, China) | Wen, Peng-Fei (China Agricultural University, Beijing, China) | Chen, Jian-Ye (China Agricultural University, Beijing, China) | Kong, Wei-Fu (China Agricultural University, Beijing, China) | Pan, Qiu-Hong (China Agricultural University, Beijing, China) | Zhan, Ji-Cheng (China Agricultural University, Beijing, China) | Tian, Li (China Agricultural University, Beijing, China) | Liu, Hong-Tao (China Agricultural University, Beijing, China) | Huang, Wei-Dong (China Agricultural University, Beijing, China), E-mail: huanggwd@263.net
To investigate whether phospholipase D (PLD, EC 3.1.4.4) plays a role in adaptive response of post-harvest fruit to environment, a PLD gene was firstly cloned from grape berry (Vitis Vinifera L. cv. Chardonnay) using RT-PCR and 3'- and 5'-RACE. The deduced amino acid sequence (809 residues) showed 84.7% identity with that of PLD from Ricinus communis. The secondary structures of this protein showed the characteristic C2 domain and two active sites of a phospholipid-metabolizing enzyme. The PLD activity and its expression in response to heat acclimation were then assayed.
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