Development of a Monitoring Vector for Leuconostoc mesenteroides Using the Green Fluorescent Protein Gene
2007
Lee, K.H. (Kyung Hee University, Suwon, Republic of Korea) | Park, W.J. (Kyung Hee University, Suwon, Republic of Korea) | Kim, J.Y. (Kyung Hee University, Suwon, Republic of Korea) | Kim, H.G. (Kyung Hee University, Suwon, Republic of Korea) | Lee, J.M. (Kyung Hee University, Suwon, Republic of Korea) | Kim, J.H. (Gyeongsang National University, Jinju, Republic of Korea) | Park, J.W. (University of Ulsan, Ulsan, Republic of Korea) | Lee, J.H. (Kyonggi University, Suwon, Republic of Korea) | Chung, S.K. (Shinheung College, Uijeongbu, Republic of Korea) | Chung, D.K. (Kyung Hee University, Suwon, Republic of Korea), E-mail: [email protected]
The vector pCW5 with plasmid pC7, originally isolated in Lactobacillus paraplantarum C7 derived from kimchi, was constructed using a p32 strong promoter, the pC7 replicon, and green fluorescent protein (GFP) as the reporter. The constructed vector was transformed into E. coli and Leuconostoc mesenteroides, and GFP expression detected using a Western blot analysis. GFP fluorescence was recognized in E. coli and Leuconostoc mesenteroides using a confocal microscope. In addition, GFP fluorescence was also clearly detected in several industrially important lactic acid bacteria (LAB), including Lactobacillus bulgaricus, Lactobacillus paraplantarum, and Lactobacillus plantarum.
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