Occurrence of aflatoxin M₁ in milk determined by HPLC with derivatization method in Korea (1999-2000)
2007
Kang, H.G. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: kanghg@nvrqs.go.kr | Cho, J.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
In this study, the levels of aflatoxin M₁(AFM₁) in milk were determined by HPLC with derivatization method. Milk samples were purified using C∧18 disposable cartridge followed by derivatization with trifluoroacetic acid and analysed using HPLC with fluorescence detection. The recoveries of AFM₁ from milk samples added AFM₁ at a level of 0.025~0.1 ng/ml were 94.7~98.0% with detection limit of 0.009 ng/ml. The amounts of AFM₁ were determined below 0.05 ng/ml for all tested samples of commercial milk collected in 1999 and 2000.
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