Cell growth and nutritive value of the tropical benthic diatom Amphora sp.at varying nutrients, light intensity and location of culture
2006
dela Pena, M.R., Southeast Asian Fisheries Development Center, Tigbauan, Iloilo 5021 (Philippines). Aquaculture Dept.
Two series of experiments were conducted to determine the suitable growth factors (nutrient medium, photosynthetic photon flux density and location of culture) for the mass propagation of the local algal isolate Amphora sp. The first series showed that cultures exposed to lower photosynthetic photon flux density (11.4 micro mole photon/sq m/s attained a higher growth rate (0.3 division/day) compared to cultures exposed to higher levels of photosynthetic photon flux densities (16.1 micro mole photon/sq m/s,-0.0 div./day, 31.3 micro mole photon/sq m/s, 0.0 div./day). The second series showed that cultures located inside the laboratory had a significantly higher cell density (133 x 10E4 cells/sq cm) compared to cultures located outdoors (100 x 10E4 cells/sq m.). Comparison of nutrient media across two locations showed that lipid content was significantly higher in cultures enriched with F/2MTM (macronutrients + trace metals) and F/2 MV (micronutrients + vitamins). The saturated fatty acids were also high in cultures enriched with F/2M (macronutrients). No significant differences were noted in unsaturated fatty acids, n-3 fatty acids, n-3 HUFA and ratios of n-3 to n-6 polyunsaturated fatty acids (PUFA). Comparison of culture locations across nutrient medium showed that lipids, n-3 fatty acids, n-3 HUFA and n-3/n-6 ratio were not significantly different. However, significantly higher saturated fatty acids were observed in cultures located outdoors (33.1%) compared to cultures located indoors (26.6 %). The proteins, carbohydrates and n-6 fatty acids content of Amphora sp. were influenced by the location and enrichment of culture. This study identified the cheaper enrichment medium, optimum photosynthetic photon flux density and location of culture for the mass propagation of Amphora sp. and determined biochemical composition at these culture conditions.
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