An in vitro study on micropropagation of Caladium bicolor
2007
Ali, A. (Punjab Univ., Lahore (Pakistan). Seed Centre) | Munawar, A. (Lahore Coll. for Women Univ., Lahore (Pakistan). Dept. of Botany) | Naz, S. (Lahore Coll. for Women Univ., Lahore (Pakistan). Dept. of Botany)
Rapid propagation of Caladium bicolor was achieved in vitro. Excellent results for shoot induction from apical meristem were obtained when it was cultured on MS medium containing 1mg L-1 BAP within 8 days of inoculation. For shoot multiplication maximum number of shoots was obtained when 0.25 mg L-1 NAA was added to shoot induction medium i.e., MS medium supplemented with 1 mg L-1 BAP + 0.25 mg L-1 NAA. Cent percent rooting was achieved by transferring an individual microshoot to modified MS medium containing combination of 2 mg L-1 IBA + 1 mg L-1 NAA. Plantlets were successfully transferred to greenhouse in sterilized sand and nourished with Hoagland's solution. After acclimatization these plants were shifted to natural conditions in pots containing growth mixture (sand + clay + peat at 1:1:1 ratio), where 100% survival was observed. We concluded that technique of micropropagation can successfully be used for large-scale production of premium quality planting material and almost 2500 plants of Caladium could be generated from single apical meristem in a 12 weeks period.
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