Detection of Methicillin/oxacillin resistant Staphylococcus aureus isolated from some clinical hospitals in Cairo using Meca/Nuc genes and antibiotic susceptibility profile
2007
Rushdy, A.A. (Ain Shams Univ., Cairo (Egypt). Botany Dept.) | Salama, M.S. (Ain Shams Univ., Cairo (Egypt). Faculty of Science) | Othman, A.S.
This study reports the detection of methicillin/oxacillin-resistant Staphylococcus aureus (MRSA/ORSA) strains and generation of antibiogram profile of S. aureus clinical isolates from three Egyptian hospitals. PCR amplification, spot inoculation and oxacillin disc diffusion tests were applied to compare their MRSA/ORSA detection abilities. From 200 bacterial isolates tested, 83 (41.5%) were confirmed as S. aureus of which 51 (61.45%) were oxacillin resistant (ORSA). Out of 51 isolates 26 had single resistance (oxacillin resistance), while 25 had double resistance (oxacillin & methicillin) resistance (MRSA/ORSA). Antibiotic susceptibility, of all isolates, to seven different antibiotic groups was tested. Quinolines and aminoglycosides were the most effective groups, 45.1% of the isolates were susceptible to vancomycin. However, 27.5% of the isolates were multidrug resistant, against many of the available antibiotics and thus there is an urgent need for alternative antibiotics. Using the PCR assay, 26 ORSA isolates were found to have nuc gene and 25 MRSA/ORSA had mec A and nuc genes. Their amplification on agarose gel revealed the presence of nuc gene at 276 by and for mec A gene at 533 bp. The sequencing of both genes was done and sequence alignment of both genes showed 99% and 97% homology between mec A and nuc genes, respectively.
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