In-vitro plantlet regeneration from shoot tip of field-grown hermaphrodite papaya (Carica papaya L. cv. Eksotika)
2007
Panjaitan, S.B. (Universiti Putra Malaysia, Serdang (Malaysia). Dept. of Agriculture Technology) | Aziz, M.A. (Universiti Putra Malaysia, Serdang (Malaysia). Dept. of Agriculture Technology) | Rashid, A.A. (Universiti Putra Malaysia, Serdang (Malaysia). Dept. of Agriculture Technology) | Saleh, N.M. (Universiti Putra Malaysia, Serdang (Malaysia). Dept. of Cell and Microbiology)
An experiment was carried out on plantlet regeneration from shoot tip of hermaphrodite field-grown papaya (Carica papaya L. cv. Eksotika). BAP concentrations in combination with NAA were assessed for shoot induction and proliferation. For rooting; pretreatment in different indol-3-butyric acid (IBA) concentrations, followed by culture on medium with and without vermiculite supplement were evaluated. MS medium containing 1.0 mg L-1 6- benzylaminopurine (BAP) and 0.05 mg L-1 alpha-naphthaleneacetic acid (NAA) produced the highest mean number of shoots (73.3) per explant. The number of emerging shoots per explant differed significantly from all other treatments including the control. A highest rooting percentage (95%) was obtained on shoots pretreated in 1.0 mg L-1 IBA. Culture on medium supplemented with vermiculite, exhibited a mean number of 1.4 roots per shoot and mean root length of 2 cm. A protocol for in-vitro plantlets regeneration from shoot tip of hermaphrodite field- grown papaya (Carica Papaya L. cv. Eksotika) is developed.
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