Biofilm formation and drinking water quality in relation to Escherichia coli at commercial poultry farms
2008
Ahmad, S. (University of Agriculture, Faisalabad (Pakistan). Dept. of Veterinary Microbiology) | Ashraf, M. (University of Agriculture, Faisalabad (Pakistan). Dept. of Veterinary Pathology) | Siddique, M. (University of Agriculture, Faisalabad (Pakistan). Dept. of Veterinary Pathology) | Mehmood, F. | Arshad, M. | Khan, H.A.
Biofilms are dense aggregates of surface adherent microorganisms embedded in a poly saccharide matrix. The most alarming is the presence and multiplication of opportunistic pathogens such as Escherichia coli found in biofilms. The present study was designed to determine the presence of E. coli in biofilm and water samples at various commercial poultry farms and to analyze factors involved in the formation of biofilm. A total of 120 biofilm and water samples were collected from inside surface of water supply network from commercial broiler and layer poultry farms in and around Faisalabad and (hang. History of the flocks was recorded on a pre-designed questionnaire. E. coli was isolated form biofilm and water samples. Out of 60 biofilms samples 58 (96.6%) and out of 60 water samples 50 (83.3%) samples was found positive for E. coli. The quantity of bacteria in the samples was determined by acridine orange direct count and viable count. Maximum acridine orange count in biofilm and water samples was recorded 2.86x0(9) and 1099x 10(7) mL-1, respectively. Maximum viable count was 1.61 x 10(9) and 1.05 x 10(3) mL-1 in biofilm and water samples, respectively. Biofilm contributes many species of bacteria but major one is E. coli.
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