Extraction of antifungal substances from Burkholderia cepacia with antibiotic activity against Colletotrichum gloeosporioides on papaya (Carica papaya)
2008
Kadir, J. (University of Putra Malaysia (Malaysia). Dept. of Plant Protection) | Rahman, M.A. (University of Putra Malaysia (Malaysia). Dept. of Crop Science) | Mahmud, T.M.M. (University of Putra Malaysia (Malaysia). Dept. of Crop Science) | Rahman, R.A. (University of Putra Malaysia (Malaysia). Dept. of Food Technology) | Begum, M.M.
An experiment was conducted to extract and determine the nature of antifungal substances produced by Burkholderia cepacia stain B23 that were inhibitory towards Colletotrichum gloeosporioides. In addition, the effect of different culture media on the production of antifungal substances by B. cepacia B23 was also investigated to have improved efficacy of this biocontrol agent. B. cepacia B23 grew faster in nutrient broth medium and the cell concentration in this liquid medium reached the highest level at 72 h after inoculation. Consequently, this strain of B. cepacia produced more antifungal substances in a nutrient broth medium than other tested media. Higher dilution (1:8) of the antifungal substances in crude supernatant from B. cepacia B23 was found to inhibit the mycelial growth and spore germination of C. gloeosporioides by 41% and 100%, respectively. Pyrrolnitrin and other antifungal compounds were detected on TLC plates, which were thermostable. Improvement in biocontrol efficacy may be possible by adjusting cultural conditions to optimize the production of antifungal substances by B. cepacia B23.
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