Application of the conductometric method for differentiation of proteins and peptides
2007
Mierzejewska, D. | Marciniak-Darmochwal, K. | Kostyra, H. | Rudnicka, B.,Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research, Dept. of Food Chemistry
The aim of this work was to use the conductometry method for the differentiation of the chemically- and enzymatically-modified food proteins. The protein extracts of soybean, bean pea, maize and rice seeds, 3 varieties Tonacja, Nawra and Sukces of wheat, as well bovine serum albumin, pepsin trypsin and acid protein hydrolyzates were investigated. Additionally, proteins were glycated by glucose. It was found that differences of the electrolytic conductivity between native proteins and their hydrolyzates were statistically significant, the electrolytic conductivity of the water protein solutions was directly proportional to the concentration of protein. It was also proved that the concentration of glucose in the protein solution modified its electrolytic conductivity. Moreover, statistically significant differences were observed between native and glycated proteins. Finally, it was stated that the electrolytic conductivity can be a tool for the differentiation of the native and modified proteins
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