Biosyntesis of xylanolytic enzymes in cultural medium of white root Phanerochaete chrysosporium
2006
Gorska, E.B.,Szkola Glowna Gospodarstwa Wiejskiego, Warszawa (Poland). Zaklad Mikrobiologii Rolniczej | Wilgat, K.,Instytut Melioracji i Uzytkow Zielonych, Falenty (Poland) | Russel, S.,Szkola Glowna Gospodarstwa Wiejskiego, Warszawa (Poland). Zaklad Mikrobiologii Rolniczej
Degradation of wood in nature is caused mainly by white rot fungus representing Basidiomycetes, such as Phanerochaete chrysosporium. The aim of this studies was to investigate the production of xylanolytic enzymes (endo- 1.4-beta-xylanase and beta-xylosydase) by Phanerochaete chrysosporium cultivated on Czapek mineral medium to which straw or wheat bran was added. The results showed that P. chrysosporium produced both enzymes endo- 1,4-beta-xylanase and beta-xylosydase in culture medium with the two substrates added. The activity of endo- 1,4-beta-xylanase and beta-xylosydase depends on temperature and it is highest at 70 and 60 deg C. The maximum activity of both enzymes was found for the acid reaction, pH = 4.6
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