Development of sex-associated SCAR markers in Piper longum L.
2005
Manoj, P. (Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala (India). Plant molecular Biology Laboratory) | Banerjee, N.S. | Ravichandran, P.
Piper longum is a medicinally important dioecious plant species of tropical India and adjoining countries. The molecular basis of dioecy in this species is not known. We have previously reported evidence for genetic differences between the male and female plants of this species through RAPD analysis. Here we describe our findings using two more male-associated RAPD markers (OPA10827 and OPA15744),and the development of one male-associated sequence characterized amplified region (SCAR) marker. Male sex-associated RAPD bands are sequenced for the development of male-associated SCAR markers in P. longum. We could convert the RAPD marker OPA10827 into a SCAR marker. The SCAR developed based on the DNA sequence of the other RAPD marker, was found to be present in some 20% of the female population of P. longum. Based on this and Southern hybridization experiments, we understand that there is considerable homology between the chromosomes of male and female genotypes from which RAPD markers were derived. Nevertheless, our experiments demonstrated that this sequence showed detectable differences between the male and female plants of dioecious P. longum
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