Characterization of wild wheat (Aegilops L.) and wild barley (Hordeum L.) germplasm using intersimple sequence repeat (ISSR) and general DNA primers.
2006
An Hang | Burton, C.S. | Bockelman, H.
Intersimple sequence repeat (ISSR) and general amplification primers were used to study the genetic diversity among 17 Aegilops and 17 Hordeum species, using 24 primers. Eight of the primers generated 102 polymorphic bands in Aegilops and all of the 24 primers produced 247 polymorphisms in Hordeum accessions. General primers were able to differentiate among Aegilops and Hordeum species. Cluster analysis of the similarity coefficients indicated that species sharing a common genome tended to group into a defined cluster(s), while species with a unique genome were obviously dissimilar. DNA analysis utilizing ISSR and general sequence DNA primers has the potential for use in phylogenetic studies of wild wheat and barley species.
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