The influence of nickel on the in vitro growth of some poplar clones from the Leuce section
2006
Katanic, M.,University of Novi Sad, Novi Sad (Serbia). Institute for Lowland Forestry and Environment | Tomovic, Z.,Forest Enterprise Vojvodinasume, Novi Sad (Serbia) | Orlovic, S.,University of Novi Sad, Novi Sad (Serbia). Institute for Lowland Forestry and Environment | Krstic, B.,University of Novi Sad, Novi Sad (Serbia). Faculty of Natural Sciences and Mathematics, Institute of Biology and Ecology
This research deals with the effect of nickel presence in growth medium on growth of poplar clones cultivated in tissue culture. Shoots of clones L-12, L-80, L-111/81 and LBM were multiplicated by micropropagation on ACM with 0.5 mg/l 6-benzylaminopurine (BAP) and 0.02 mg/l alfa-naphthaleneacetic acid (NAA). Therminal shoots, about 1.5 cm long, were cultivated 25 days in the ACM medium with addition of 0.1 mM and 0.5 mM of Ni. Parameters investigated in shoot cultures included: length of the main shoot, formation of axillary shoots, fresh and dry mass. It was observed that the addition of 0.5 mM Ni was toxic for shoots of all investigated clones. The symptoms included were decrease of growth vigour and leaf chlorosis. Length, fresh and dry mass, shoot multiplication also decreased compared to the control. Low concentration of nickel caused increased length of shoots of clone L-12 and L-111/81. Also, shoots of clone L-111/81 manifested increased fresh and dry mass, but differences were not statistically significant. These results show that lower concentration of nickel can cause growth stimulation of the clone L-111/81, which indicates its ability for nickel phytoremediation.
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