Morphological and immunohistochemical examination of tumor cells in Marek disease
2007
Pejovic, N., Biotechnical Institute, Podgorica (Montenegro) | Velhner, M., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Polacek, V., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Aleksic-Kovacevic, S., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Marinkovic, D., Faculty of Veterinary Medicine, Belgrade (Serbia) | Knezevic, M., Faculty of Veterinary Medicine, Belgrade (Serbia)
In this paper are described the macroscopic, microscopic and immunophenotypic characteristics of tumors in Marek disease (MD). Liver, kidneys, lungs, heart, proventriculus, ovary and nerves of sciatic plexus collected from 20 chickens aged 8 to 24 weeks were examined. Lymphoproliferative enlargement in various organs of focal and diffuse character was found. The affected organs were enlarged, compact and very brittle, irregular in shape, grayish-red or grayish-white and fatty consistency. Most often characteristic changes were in the spleen, liver, proventriculus and ovary that usually had a compact or ribbed, fatty cauliflower like formation, different in size. On histological examination proliferation of small and medium size lymphocytes, lymphoblast cells, Marek disease cells and activated reticulum cells were found. Tumour proliferates prodominantly consisted of lymphoblasts and had all the characteristics of a lymphoma. Immunohistochemical analysis revealed that lymphoma cells in MD are of CD3 + phenotype. In liver proliferates numerous CD79 + cells were found perivasculary, which is an interesting finding demanding further research.
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