The isoenzymic analysis of cabbage genotypes (Brassica oleracea var. capitata L.)
2007
Nikolic, Z., Nacionalna laboratorija za ispitivanje semena, Novi Sad (Serbia) | Milosevic, M., Nacionalna laboratorija za ispitivanje semena, Novi Sad (Serbia) | Cervenski, J., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Gvozdenovic, Dj., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia)
The present study demonstrates the potential of isoenzymes and their application in marker assisted selection. Twelve cabbage genotypes, eight experimental lines and four varieties derived at the Vegetable Department of the Institute of Field and Vegetable Crops in Novi Sad (Serbia) were studied. The method of horizontal starch-gel electrophoresis was used to analyse enzymic systems: malate dehydrogenase (MDH), phosphoxerose isomerase (PHI), phosphoglucoso dehydrogenase (PGD), phosphoglucomutase (PGM), and alcohol dehydrogenase (ADH). PHI and PGD enzymes were polymorphic, with different alleles. Different phenotypes in cabbage varieties and genetic uniformity in lines were obtained. These results could be practically applied in the process of breeding, i.e. in the selection of a parent line combination relating to the process of the cabbage hybrid development. Accordiing to the results obtained in the isoenzymic study of the experimental material and cabbage varieties it can be concluded that enzyme polymorphism can be used for the estimation of genetic purity of both, line and hybrid seeds.
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