Genetic heterogeneity of bovine viral diarrhoea virus (BVDV) strains isolated in Slovenia
2002
Toplak, I., Ljubljana Univ. (Slovenia). Veterinary Fac. | Barlic-Maganja, D. | Hostnik, P. | Grom, J.
A selection of 44 bovine viral diarrhoea viruses (BVDV), isolated mainly from persistently infected cattle on 24 Slovenian farms between 1997 and 2001, were genetically characterised. The alignment and genetic typing of the 5’NCR (5’ noncoding region) sequences of all the Slovenian isolates showed that several of them were identical, giving a total number of 14 different sequences. In Europe, eleven subtypes of the BVDV-1 genotype have recently been identified and the Slovenian isolates were clustered into five of these phylogenetic groups. Seventeen isolates were grouped into BVDV 1d, twenty-one into BVDV 1f, four were grouped into BVDV 1b, one isolate belonged to the genetic group BVDV 1a, and another to BVDV 1g. No genotype-2 BVDV was found. This genetic prevalence matched those previously reported for neighbouring European countries, as opposed to findings reported for e.g. France, Spain, the UK and the USA. Several virus isolates from eight cattle herds were analysed and, with one exception, all the isolates were of the same genetic group. A comparison between the 5’NCR sequences of BVDV isolates from different herds has the advantage of being an extremely rapid method of confirming the virus genotype and gives further indication of a viral relationship between two or more herds.
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