Localization of a new resistance gene against powdery mildew in Hordeum vulgare ssp. spontaneum by means of DNA markers
2005
Teturova, K.(Masaryk Univ., Brno (Czech Republic). Faculty of Sciences, Department of Genetics and Molecular Biology | Repkova, J. | Lizal, P. | Dreiseitl, A.Zemedelsky vyzkumny ustav s.r.o. Kromeriz, (Czech Republic))
A newly identified accession of wild barley (Hordeum vulgare ssp. spontaneum) resistant to powdery mildew caused by Blumeria graminis f. sp. hordei was studied with the aim of finding the number of genes conferring the resistance to powdery mildew, their allelism with the Mla locus, and the other aim was to localize individual genes on barley genetic map. The genetic as well as the molecular analyses were performed in the segregating F2 population of the cross between the winter barley cultivar Tiffany and the resistant accession PI466197. Microsatellite DNA markers from known databases were used for the localization of resistance genes on barley chromosomes. The amplification methods for each of 117 DNA markers were optimized and polymorphism between the parents (Tiffany vs. H. spontaneum accession) was investigated and analysed by agarose and polyacrylamide gel electrophoresis. Polymorphism was displayed for 64% microsatellites. Modified bulk segregant analysis was used for the identification of markers linked with resistance genes. The genetic analysis showed that in this accession the resistance was determined by two independent genes with dominant mode of inheritance. Allelism test confirmed that one resistance gene was in Mla locus. The molecular analysis revealed highly significant linkage with the markers Bmac0213 and MGB402 on the short arm of chromosome 1H. This position is consistent with the Mla locus. The other gene was proved to be high significantly linked with Bmac0134 and MWG878 on the short arm of chromosome 2H. This is the promising newly identified locus. The prospect of our work is the identification of further more tightly linked DNA markers and fine mapping of resistance genes.
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