Exochitinases and exo-beta-1,3-glucanases synthesized in the barley primary leaves infected by powdery mildew in the period of pathogen sporulation
2005
Hlinkova, E.(Univerzita Komenskeho, Bratislava (Slovak Republic). Prirodovedecka fakulta | Perecko, D.Slovenska akademia vied, Bratislava (Slovak Republic). Ustav molekularnej biologie | Kovacova, Z.Univerzita Komenskeho, Bratislava (Slovak Republic). Prirodovedecka fakulta | Rafay, J.Vyskumny ustav zivocisnej vyroby, Nitra (Slovak Republic) | Bobak, M.Univerzita Komenskeho, Bratislava (Slovak Republic). Prirodovedecka fakulta | Kutarnova, Z.Univerzita Komenskeho, Bratislava (Slovak Republic). Prirodovedecka fakulta)
Present work studied changes in the content of selected hydrolytic enzymes induced in the patterns of basic proteins synthesized in the barley primary leaves infected by powdery mildew with different virulence possibility. Protein patterns analyses show that basic intercellular protein spectra strongly reflect of interaction phenotype. Incompatible and hypersensitive interaction of hosts showed relatively the smallest changes in the both quantitative and qualitative changes in the protein content in the period of pathogen sporulation. By compatible interactions, content of basic beta-1,3-exoglucanases changed their quantity as well as their quality. The most expressive changes were in the region of Mr=40-45 kDa where are localized de novo synthesized basic glucanases. Four basic exo- glucanases were synthesized constitutly independently of the host resistance genes and the pathogen avirulence/virulence genes. Their Mr were from molecular mass interval 72-45 kDa. De novo synthesized exochitinases were two. Low molecular basic form of exo Chi 14.4 was not serologicaly related to their acid form, or it is possible that the quantive content of this protein is under level of imunoblot sensitivity.
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