Development of infection caused by bacteria Erwinia carotovora subsp. atroseptica in stored potato tubers (Solanum tuberosum L.)
2005
Heldak, J.(Potato Research and Breeding Inst., Velka Lomnica (Slovak Republic) | Forisekova, K. | Debreova, K.
Tubers of artificially infected potato plants were inspected visually, tested by ELISA and PCR method. All Erwinia carotovora isolates used in these experiments gave positive results using ELISA and PCR. Symptoms of soft rot on tubers of all varieties tested after harvest varied between 7-15%. Number of diseased tubers with symptoms increased during one-month storage about 6.5%. Standard ELISA was found to be unreliable to detect bacteria Erwinia carotovora. Number of Erwinia carotovora infected tubers was equal to the number of tubers with visual symptoms on tubers after harvest and one-month storage respectively. Considerably different results were recorded in the case of Erwinia carotovora subsp. atroseptica specific PCR assay. Number of tubers analysed after harvest in which presence of bacteria Erwinia carotovora subsp. carotovora was detected by PCR varied between 19-30.6% depending on variety. During one-month storage the amount of Erwinia carotovora subsp. carotovora infected tubers increased almost two times. Tubers with positive PCR assay for Erwinia carotovora subsp. atroseptica and no visual symptoms were planted in glasshouse and 34.6% did not displayed any symptoms on stems or tubers. It is not possible to explain fully the reasons for the absence of any symptoms.
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