Genetic diversity of a black poplar population in the Morava river basin assessed by microsatellite analysis
2004
Pospiskova, M. | Bartakova, I.
Black popIar (Populus nigra L.) is an important pioneer tree species of riparian ecosystems, but it is endangered due to loss of its natural habitats in the west and central Europe at present. The gene tie structure of the population from the Morava river basin in the Czech Republic was investigated by microsatellite analysis. 112 adult trees (50-140 years old) were characterised at 12 microsatellite loci. There were identified 8 groups of identical genotypes (altogether 24 trees) among these individuals. All tested loci were highly polymorphic with a mean number of alleles 13.4 per locus, but almost 17 % of alleles were rare, appearing only once. The high values of expected heterozygosity and effective number of alleles (0.83 and 6.5, respectively) indicate high diversity in the population as well as the high value of observed heterozygosity (0.79) shows large number of heterozygous individuals. The positive mean of single-locus fixation index values indicates a weak overall excess of homozygotes. In order to study the spatial structure and gene flow within the population, four individual study sites were considered as subpopulations. Low FsT values confirm the existence of gene flow among study sites and small genetic differentiation among them. However the calculated variation parameters could be loaded by considerable sampling error, since the numbers of trees in the individual study sites were rather small.
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