Occurrence of arcobacters in the Czech Republic and the influence of sample matrix on their detection using PCR
2006
Pejchalova, M. | Vytrasova, J. | Brozkova, I. | Huskova, Z. | Cervenka, L.
Sixty nine meat and water samples were examined by multiplex-PCR (m-PCR) assay of the enrichment media. Twenty nine (42%) samples were contaminated with Arcobacter species. In 17 samples (24.6%), A. butzleri was confirmed by m-PCR. Direct detection of arcobacters from assay avoided cultivation on agar plates and reduced the time required for detection. Sensitivity of optimised m-PCR method was verified using four different enrichment broths artificially contaminated with pure cultures of arcobacters. Enrichment medium Formula 6 (composed of a special peptone No. 2, meat extract, yeast extract, sodium chloride, NaH2PO4, K2HPO4, defibrinated sheep blood, pyruvate, mercaptoacetic acid and cefoperazone) gave the best results of all tested recommended enrichment media. Influence of matrix samples was tested on genuine samples of chicken, pork and beef, in which suspension of definite density of arcobacters was added. It was shown, particularly, that a sample with higher fat content has required a longer incubation period in enrichment broth for successful detection by m-PCR assay.
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