Enterotoxin production in Staphylococcus sp. isolated from sheep milk
2007
Vasiľ, M., University of Veterinary Medicine, Košice (Slovak Republic) | Fotta, M., University of Veterinary Medicine, Košice (Slovak Republic) | Elečko, J., University of Veterinary Medicine, Košice (Slovak Republic)
Aim of this work was the determination of enterotoxin producing strains, their frequency and comparison of enterotoxin production ability to the production of nucleases in Staphylococcus sp. isolated from sheep milk. 1275 samples of sheep milk originating from two farms were examined for positivity to staphylococcus during the period from May to October 2005. From sheep milk a total of 125 strains of Staphylococcus sp. were isolated of which 117 strains were found to be capable of producing staphylococcal enterotoxins. Out of the 117 strains of Staphylococcus sp. isolated from sheep milk 23 produced staphylococcal enterotoxins. Staphylococcal enterotoxin type C (SEC) occurred most frequently (14 cases, i.e., 60.9%) where as 2 cases were identified for SEA, SED and SEE each. Production of DNA-se and TNA-se in all coagulase-positive strains of Staphylococcus sp., i. e. in Staphylococcus aureus was confirmed. Coagulase-negative strains of Staphylococcus sp. did not produce TNA and DNA, although they produced the enterotoxins. It refers to the fact that production of enzymes proves the presence of Staphylococcus aureus in examined samples, but does not confirm the presence of Staphylococcus enterotoxins as was expected.
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