An improved method for production of doubled haploid lines of winter oilseed rape
2007
Klíma, M., Výzkumný ústav rostlinné výroby, v.v.i, Praha – Ruzyně (Czech Republic) | Vyvadilová, M., Výzkumný ústav rostlinné výroby, v.v.i, Praha – Ruzyně (Czech Republic) | Kučera, V., Výzkumný ústav rostlinné výroby, v.v.i, Praha – Ruzyně (Czech Republic)
Microspores of three winter rape F1 hybrids were cultivated in the presence of antimitotic chemicals colchicine, trifluralin and oryzalin to evaluate their effect on frequency of embryo formation, embryo development, plant regeneration and diploidization rate. Ploidy level evaluation of 1709 flowering microspore derived regenerants demonstrated that in vitro treatment with all antimitotic chemicals increased significantly the rate of doubled haploid (DH) plants. Mean rate of DH plants after application of trifluralin was 85.7%, colchicine 74.1% and 66.5% after application of oryzalin, while the rate of spontaneous diploidization was only 42.3%. The application of colchicine to in vivo regenerants did not increase the mean rate of DH plants (55.6%). Although there were no significant differences in diploidization efficiency between in vitro applications of particular antimitotic agents, trifluralin showed to be the most suitable because of its positive effect on embryo development and conversion. In addition, the diploidization rate was sufficient and stable in all genotypes tested. According to our results, application of trifluralin to microspore culture could be an effective chromosome doubling tool for efficient production of doubled haploid lines from breeding materials of winter oilseed rape.
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