Factors related to multiplication rate of Garcinia succifolia Kurz. In vitro
2001
Rangsima Amphawan(Maejo University, Chiang Mai (Thailand). Office of Agricultural Research and Extension) | Nopmanee Topoonyanont(Chiang Mai University, Chiang Mai (Thailand). Faculty of Science. Department of Biology) | Tipsuda Pookmanee(Maejo University, Chiang Mai (Thailand). Office of Agricultural Research and Extension) | Sirikul Kesa(Chiang Mai University, Chiang Mai (Thailand). Faculty of Science. Department of Biology)
Explants from the shoot tips resulting from induction of seeds of young fruit of Sompong tree (Gracinia succifolia Kurz.) were cultured on MS media (1962) which contained 1.0 mg/l BA+0.3 mg/l NAA. These explants were multiplied in vitro using WPM medium that contained BA at various concentration. The study showed that 2.0 mg/l IBA caused shoot tip explants to produce buds in the shortest period of time (3.06 days) compared to the other treatments at an average of 3.02 axillary buds per explants. Although Som-pong was multiplied at a high rate as indicated by a high number of axillary buds produced, yet only a few buds were able to grow and develop. Hence the improvement of plant quality prior to root induction occurred through the production of shoot tip explants from axillary buds in 0.5 mg/l BA+0.2 mg/l IBA supplemented with 1.0 mg/l riboflavin. This increased the elongation of new axillary buds to 0.42 cm that was twice better than without riboflavin. Afterwards when the concentration level of BA was reduced to 0.25 mg/l, it was able to increase the elongation of explants together with the increase in size of the leaves. The explants from the axillary buds also increased their length more than the explants from shoot tip. At the same time, explants from shoot tip showed the increase of the size and number of leaves was higher than the explants from the axillary buds.
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