Chromosome investigation of local variety and hybrids of Amaryllis
2001
Praphatsorn Arayakitcharoenchai(Chiang Mai University, Chiang Mai (Thailand). Faculty of Agriculture. Department of Horticulture) | Chuntana Suwanthada(Chiang Mai University, Chiang Mai (Thailand). Faculty of Agriculture. Department of Horticulture)
Suitable technique of root tip preparation for somatic chromosome investigation of amaryllis via squash method was described. Root tip samples were best taken during 09.30-10.00 a.m. The tissue was then pretreated in PDB for 48 hours at 10 deg C prior to 5 minutes of fixation in fixative solution. Maceration was done in 1 N HCl solution for 5 minutes. Tissue staining in carbol fuchsin for 24 hours was carried out before squashing. Chromosome investigations of the red small-flowered local variety (R), Apple Blossom (P), Orange Sovereign (O) and their hybrids revealed the chromosome numbers being 2n=22 in R, 2n=44 in P and O, 2n=33 in RxP, 2n=34 in RxO and 2n= 36 in OxR.
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