Minimal bactericidal concentration of pomegranate (Punica granatum L.) peel extract on salmonella spp. isolated from chickens and comparison of its effects to antimicrobial agents
2006
Kanlaya Chuachan(Khon Kaen University, Khon Kaen (Thailand). Faculty of Veterinary Medicine. Dept. of Medicine) | Jiroj Sasipreeyajan(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Sciences. Department of Veterinary Medicine) | Nantavan Bunyapraphatsara(Mahidol University, Bangkok (Thailand). Faculty of Pharmacy. Dept. of Pharmacognosy)
Dry pomegranate (Punica granatum L.) peel were powdered and extracted by maceration in ethyl alcohol (95 percent). Then the extract was filtered and evaporated under vacuum. This crude extract exhibited microbiocidal action on 70 isolates of Salmonella spp. isolated from chickens. The minimal bactericidal concentration (MBC) determined by macrobroth (tube) dilution method was 1.25-2.5 percent (w/v). Seventy-seven isolates showed MBC at 1.25 percent. Antimicrobial sensitivity test against Amoxycillin, Colistin sulphate, Doxycycline hydrochloride, Gentamicin, Neomycin, Norfloxacin, Amoxycillin+Clavulanic acid and Sulphamethoxazole+Trimethoprim, showed that 6 out of 70 samples were resistant to at least one agent. The pomegranate peel extract at the concentration 1.25 percent was able to destroy 5 out of these resistant samples.
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