Changes of intensities of reactive oxygen species (ROS) free radicals from laccase-treated woods
2007
Duan Xinfang, Cao Yuanlin, Cao Yongjian
In order to investigate the changes of free radicals in the suspension liquid of wood treated by laccase, wood powder of Pinus kesiya var. langbianensis sapwood, heartwood and Liquidambar formosana were selected to detect the changes of free radicals after laccase treatment. The laccase treatment parameters were as follows: pH 3.47 and 6.50, 2 hours of laccase incubation, the temperature of 32 ℃ and enzyme dosage 19 U•g -1 dry wood powder. The results showed that there was a significant difference of the intensities of the reactive oxygen species (ROS) free radicals in the aqueous suspension of wood between control and samples treated by laccase. The intensities of ROSfree radicals among different woods treated by laccase was marked difference. The intensities of ROS free radicals of Pinus kesiya var. langbianensis sapwood or heartwood treated by laccase were higher than that of Liquidambar formosana.
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