Cloning and characterization of a new male sterility-related gene BcMF1 in Brassica campestris L. ssp. chinensis Makino
2008
Wang Huaxin | Wang Yongqin | Cao Jiashu
中国人. 筛选与白菜雄性不育性状相关的新基因，为研究植物雄性不育的分子机制提供依据。利用cDNA-AFLP技术分析白菜核雄性不育两用系‘ZUBajh97-01AB ’的表达差异，在可育株群中扩增出1条特异条带BcMF-A15T17，通过RACE和PCR技术扩增得到该基因的cDNA和DNA全长序列，并用Northern杂交验证了该基因的表达特征。该基因被命名为BcMF1，它的cDNA全长为1 684 bp，基因全长为1 985 bp，BcMF1基因仅在两用系可育株群的中、大花蕾中特异表达。推测的BcMF1蛋白含有471个氨基酸，与拟南芥未知功能蛋白家族DUF1216成员的相似性较高，预测该蛋白是一个定位在胞外的分泌蛋白。BcMF1基因是一个与白菜细胞核雄性不育相关的新基因。
显示更多 [+] 显示较少 [-]英语. This paper aims to screen a novel gene related with the male sterility in Chinese cabbage for studying the molecular mechanism of plant male sterility. The gene differential expression analysis was performed by cDNA-AFLP in the genic male sterile-fertile line ZUBajh97-01AB of Chinese cabbage, and a differentially expressed cDNA fragment, BcMF-A15T17, was found in fertile line. The full cDNA and DNA sequences of the gene related with BcMF-A15T17 were amplified by RACE and PCR, and the expression characteristics of the gene were proved by Northern blot analysis. The gene, designated as BcMF1, was 1 684 bp long in cDNA and 1 985 bp long in DNA, and specially expressed in middle and big floral buds of the fertile line. The hypothetical protein BcMF1 including 471 amino acids, which was predicted being a extracellular protein in secretory pathway, had higher similarity in the amino acids sequence to some members from a unknown protein family DUF1216 in Arabidopsis thaliana. All the results in this study suggest that BcMF1 is a new gene related with genic male sterility in Chinese cabbage.
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