New studies about the molecular resistance of tobacco plants to blue mold (Peronospora tabacina Adams)
2009
Chacón, O., Instituto de Investigaciones del Tabaco, La Habana (Cuba) | Canales, E., Centro de Ingeniería Genética y Biotecnología, Ciudad de la Habana (Cuba) | Portieles, R., Centro de Ingeniería Genética y Biotecnología, Ciudad de la Habana (Cuba) | Hernández, I., Centro de Ingeniería Genética y Biotecnología, Ciudad de la Habana (Cuba) | López, Y., Centro de Ingeniería Genética y Biotecnología, Ciudad de la Habana (Cuba) | Crespo, J.A., Instituto de Investigaciones del Tabaco, La Habana (Cuba) | Borrás, O., Centro de Ingeniería Genética y Biotecnología, Ciudad de la Habana (Cuba)
The noncultivated tobacco Nicotiana megalosiphon has been shown to be highly resistant to Blue Mold disease. This is produced by the Oomycete Peronospora tabacina Adams, which is one of the most important pathogens that affect tobacco yield in Cuba. Nowadays, resistance genes activated against this pathogen have not been isolated and there are poor knowledgement about their biochemical and signaling pathways. In order to identify tobacco (Nicotiana megalosiphon) genes involved in broad-spectrum resistance to Peronospora tabacina Adams, suppression subtractive hybridization (SSH), was used to generate cDNA from transcripts that are differentially expressed during an incompatible interaction. After differential screening by membrane-based hybridization, clones corresponding to 182 differentially expressed genes were selected, sequenced, and analyzed. The cDNA collection comprised a broad repertoire of genes associated with various processes. Some of them were involved in the signaling process. Others were related with the defence responses to pathogens attack. Also, it was used a set of SCAR markers linked to blue mold resistance to identify resistant tobacco cultivars to Blue Mold. Potentially, these results could aid in the development of blue mold-resistant tobacco cultivars.
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