Factors influencing shoot multiplication of lotus (Nelumbo nucifera)
2008
Shou, S.Y.,Zhejiang Univ., Hangzhou (China). Coll. of Agriculture and Biotechnology | Miao, L.X.,Zhejiang Univ., Hangzhou (China). Coll. of Agriculture and Biotechnology | Zai, W.S.,Zhejiang Univ., Hangzhou (China). Coll. of Agriculture and Biotechnology | Huang, X.Z.,Zhejiang Univ., Hangzhou (China). Coll. of Agriculture and Biotechnology | Guo, D.P.,Zhejiang Univ., Hangzhou (China). Coll. of Agriculture and Biotechnology
Effect of plant growth regulators, explant size, season of explant collection, temperature (20, 25 and 30 deg C) and photoperiod on in vitro lotus (Nelumbo nucifera) shoot formation and growth were examined. Shoots formation was greatly influenced by growth regulators, explant size and season of explant collection. The maximum number of shoots were induced from bud explants on Murashige and Skoog medium containing 4.44 microM benzyladenine + 0.54 microM alpha-naphthalene acetic acid. Explants formed by bud of one expanded and one unexpanded leaf which was collected in spring gave encouraging results of shoot production. Higher temperature favoured shoot induction and subsequent growth was much better at 25 deg C compared to that at 20 and 30 deg C.
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