A new probiotic and bacteriocin-producing strain of Enterococcus faecium EF9296 and its use in grass ensiling
2008
Marcinakova, M.,Slovenska Akademia Vied, Kosice (Slovak Republic). Ustav Fyziologie Hospodarskych Zvierat | Laukova, A.,Slovenska Akademia Vied, Kosice (Slovak Republic). Ustav Fyziologie Hospodarskych Zvierat | Simonova, M.,Slovenska Akademia Vied, Kosice (Slovak Republic). Ustav Fyziologie Hospodarskych Zvierat | Strompfova, V.,Slovenska Akademia Vied, Kosice (Slovak Republic). Ustav Fyziologie Hospodarskych Zvierat | Korenekova, B.,Univerzita Veterinarskeho Lekarstva, Kosice (Slovak Republic) | Nad, P.,Univerzita Veterinarskeho Lekarstva, Kosice (Slovak Republic)
A new probiotic, bacteriocin-producing strain of Enterococcus faecium EF9296 was used in grass ensiling as an inoculant. Antimicrobial effect, nutritive quality and aerobic stability of the silage were evaluated. Experimental and control silages were processed in 1-litre plastic bottles, samples were collected at days 0-1, 7, 14 and 105. The silages were subjected to an aerobic stability test for 7 days at the end of the ensiling period. The EF9296 strain survived and colonized the silage in sufficient counts. A reduction in E. coli, enterobacteriae, staphylococci and Bacillus-like bacteria was noted mainly at day 7 and 14 of ensiling. Total counts of enterococci and lactic acid bacteria increased. Listeria and moulds counts were reduced during the aerobic stability test. The silage inoculation resulted in a more rapid drop of pH, higher level of lactic acid and lower levels of acetic and butyric acids. The effect of E. faecium EF9296 on the value of pH was prolonged until the end of the experiment. An increase in dry matter content was detected. The E. faecium EF9296 strain represents a promising silage additive to produce good quality silages and/or to prevent silage contamination.
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