The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae

Lobova, D.,Veterinarni a Farmaceuticka Univ., Brno . Ustav Mikrobiologie a Imunologie; Cizek, A.,Veterinarni a Farmaceuticka Univ., Brno . Ustav Mikrobiologie a Imunologie; Celer, V.,Veterinarni a Farmaceuticka Univ., Brno . Ustav Mikrobiologie a Imunologie

The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae

2008

Lobova, D.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Mikrobiologie a Imunologie | Cizek, A.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Mikrobiologie a Imunologie | Celer, V.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Mikrobiologie a Imunologie

Single-chain antibodies (scFv) specific to Brachyspira hyodysenteriae were isolated from a phagemid library. Recombinant Bhlp 29.7 protein was used for scFv selection and individual clones were tested by ELISA and immunofluorescent test; four unique clones were isolated. One of the selected clones was able to bind specifically B. hyodysenteriae in ELISA and immunofluorescence test. This is the first report of species-specific recombinant antibodies against B. hyodysenteriae.

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