Differential expression of LEA proteins in two genotypes of mulberry under salinity
2009
Jyothsnakumari, G.,Acharya Nagarjuna Univ., Nagarjuna Nagar (India). Dept. of Botany | Thippeswamy, M.,Sri Krishnadevaraya Univ., Anantapur (India). Dept. of Botany | Veeranagamallaiah, G.,Sri Krishnadevaraya Univ., Anantapur (India). Dept. of Botany | Sudhakar, C.,Sri Krishnadevaraya Univ., Anantapur (India). Dept. of Biotechnology
The relative water content (RWC), cell membrane integrity, protein pattern and the expression of late embryogenesis abundant proteins (LEA; group 1, 2, 3 and 4) under different levels of salt stress (0, 1.0, 1.5 and 2.0% NaCl) were investigated in Morus alba cultivars (S1 and ATP) with contrasting salt tolerance. RWC and membrane integrity decreased with increase in NaCl concentration more in cv. ATP than in cv. S1. SDS-PAGE protein profile of mulberry leaves after the NaCl treatments showed a significant increase in 35, 41, 45 and 70 kDa proteins and significant decrease in 14.3, 18, 23, 28, 30, 42, 47 and 65 kDa proteins. Exposure of plants to NaCl resulted in higher accumulation of LEA proteins in S1 than ATP. The maximum content of LEA (group 3 and 4) was detected in S1 at 2.0% NaCl, which correlates with its salt tolerance.
显示更多 [+] 显示较少 [-]