Microsatellite analysis of the genetic distance between 15 potato (Solanum tuberosum L.) genotypes
2006
Tesfaye Abebe | C.D. Viljoen | M.T. Labuschagne
Advanced breeding materials and commercial cultivars that possess a number of economically important attributes are good potential building blocks of a breeding program. Their efficient utilization, nevertheless, depends on the knowledge of the genetic distance existing between them. A total of 15 potato genotypes consisting of 11 advanced breeding clonal materials at multi-locational performance trial stage and four Ethiopian converted commercial cultivars of European origin were evaluated using the molecular marker called Simple Sequence Repeat (SSR) or microsatellites to determine the genetic distance between them. Three primers flanking to the gene associated with the potato wound induced genes WIN1 and WIN2 and starch synthase were used to detect for variation in repeat length. The three primer pairs did reveal polymorphism, detecting between four and six alleles at each locus. Of these, two gave rise to complex band patterns, suggesting that multiple polymorphic loci were being amplified using a single primer pair. Consequently, 74 clearly scorable bands were recorded upon which a diversity index (H), a genetic distance matrix and cluster analysis were performed. The hetrozygosity values ranged from 0.63 to 0.82. Similarly, the genetic distance matrix ranged between 0.25 through 0.89. The lowest genetic distance was observed for the genotypes CIP-387676.24 and Tolcha whilst the maximal distance was between KP-90134.5 and CIP-387676.24. Phenetic analysis of the derived information allowed the construction of a dendrogram depicting the genetic distance between the studied genotypes. Consequently, two main clusters and three singletons were formed. The potential of microsatellite markers for genetic distance analysis and parental material selection is discussed in the light of these results.
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