A comparative study of variation in rice plants regenerated from different sources of protoplasts
2003
Pattanayak, A., ICAR Research Complex for North Eastern Region, Umiam (India | Sarma, C.M., Gauhati University, Guwahati (India). Dept. of Botany | Sarma, B.K., ICAR Research Complex for North Eastern Region, Umiam (India)
Plant regeneration from protopl';ts is always associated with protoclonal variation. Protoclones were obtained from immature embryo, mature embryo and anther-derived calli. Significant protoclonal variations, across genotypes, were seen in the RO. Plant height, panicle length, spikelets/ panicle and spike let fertility showed significant reduction in RO. 1000-grain weight did not show any significant change. In the Rl generation no significant difference. from seed-derived control was seen in the number of panicle bearing tillers (PBT) and 1000 grain weight. Panicle length and yield /plant did not show much variation across the genotypes. Spikelets /panicle and spike let fertility percentage were most affected and showed negative shift. Panicle length remained largely unaffected but spikelets/ panicle and spike let fertility showed negative shift. Uniform reduction in the plant height, panicle length and yield/plant along with spikelets/panicle and spikelet fertility across the generations, indicated additive" expressionofthese characters and stable inheritance of this trait. Although immature embryo- I derived calli are good source of cell suspension and protoplasts, variations in immature embryoderived protoclones were higher than those seen in protoclones derived from seeds. Antherderived calli showed a much higher frequency of variation in their protoclones. From the results of the present investigation it can be concluded that suspension cells derived from seeds may be a better source of pro top lasts if somaclonal variations are not desirable.
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