Transformation of potato using cryIAb gene to produce potato tuber moth resistant transgenic plants
2007
Rahnama, Hassan | Shams, Mohammad Reza
Potato is one of the most important crops in Iran. Optimization of tissue culture in order to achieve high frequency regeneration is the first step for genetic transformation. Here, the regeneration of two important potato cultivars that are widely cultivated in Iran (i.e. Agria and Marfona) was investigated. Internodes, leaves, and microtubers explants of potato were treated with different hormone components. It was found that, in the two step medium A, regeneration efficiency of internodes in 1mg/l TDZ is higher than 0.5 mg/l and 2 mg/l TDZ, whereas, no leaf explants in these media was regenerated. In the one step medium C, regeneration percentage of internodes, leaves, and microtubers has shown more efficient and powerful (i.e. rapid and high percentage of regeneration in all three type of explants) than medium A. Then we recommended the medium C as an efficient medium for genetic transformation of these cultivars of potato. On the other hand, we constructed two vector system containing cryIAb gene suitable for using in Agrobacterium mediated transformation systems. In pBinCryIAb, cryIAb gene is under the control of CaMV35S promoter. In the second vectore, cryIAb gene is under the control of PEPC promoter, that expresses the gene in green tissues of plant. These vectors were transformed to Agrobacterium tumefacience AGLO in order to be used in plant transformation.
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