Studies on designing an ELISA system for measuring nanogram
2007
Madani, Rasool | Taghavian, | Golchin Far, | Hamzehlo, Zohreh
Many formulations of media contain either bovine serum or other more purified fractions of serum proteins such as albumin which may contain varying amounts of bovine IgG. When the intended product may be used as a therapeutic agent in humans or animals the product should be highly purified to avoid potential health risks or other problems which might result from trace contaminants. Therefore an immunocapture ELISA is designed by using a rabbit polyclonal antibody, for the detection and estimation of bovine serum albumin (BSA) contamination of culture mediums. The test depends on the capturing of the antigen, BSA, by rabbit polyclonal antibody and detection by the same antibody coupled to an enzyme. The results showed this ELISA test is highly sensitive to detect contamination to less than 5 ng/ml and therefore suitable for routine detection of BSA in final viral vaccines samples.
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