Identification of drought tolerance candidate genes in sugar beet
2006
Hoseyni Salekdeh, Qasem | Haj Heydari, Mohsen | A`bdollahiyan Nuqabi, Mohammad | Sadeqiyan, Ya`qub
Drought and limits to irrigation cause sugar beet (Beta vulgaris L.) yield losses in Iran. The identification of candidate genes for marker-assisted selection (MAS) could greatly improve the efficiency of breeding for increased drought tolerance. Drought-induced changes in the proteome could highlight important genes. Two genotypes of sugar beet (7112 and 7912) differing in drought response were cultivated in the field. A line-source sprinkler irrigation system was used to apply irrigated and water deficit treatments beginning at the four-leaf stage. At 157 days after sowing, leaf samples were collected from well watered and drought stressed plants for protein extraction and to measure shoot biomass and leaf relative water content. Changes induced in leaf proteins were studied by two dimensional gel electrophoresis and quantitatively analyzed using image analysis software. Out of more than 500 protein spots reproducibly detected and analyzed, seventy-nine spots showed significant changes under drought. Some proteins showed genotype-specific patterns of up- or down regulation in response to drought. Twenty-one protein spots were analyzed by liquid chromatography tandem mass spectrometry (LC/MS/MS), leading to identification of Rubisco and 10 other proteins involved in redox regulation, oxidative stress, signal transduction, and chaperone activities. Some of these proteins could contribute a physiological advantage under drought, making them potential targets for MAS.
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