Morphology and pathogenicity of Eimeria sp. first isolated from guinea fowl in Japan
2008
Yano, K.(Tokyo Univ. of Agriculture, Atsugi, Kanagawa (Japan). School of Animal Science) | Murakami, S. | Inoue, I. | Watanabe, T. | Ogawa, A. | Matsui, T.
Coccidium isolated from the feces of a guinea fowl was purified by the single-oocyst isolation method. The sporulated oocysts were ovoid, on average 24.0 x 18.7 microm in size with small polar granules, but no micropyle or micropyle-cap was observed. Sporocysts were ellipsoid, on average 9.5x6.1microm in size with a small Stieda body and a residual body. To identify the species and clarify their life cycle and pathogenicity, we orally administered the oocyst to three-day-old guinea fowl chicks. The prepatent period was five days. Although all examined birds survived during the experiment period, watery diarrhea was observed in these birds. Histopathologically, the small intestine and caeca revealed catarrhal enteritis, and schizogony was confirmed in the small intestine and gametogonic stages in the caeca. These results did not coincide with the stages of Eimeria numidae and E. grenieri of guinea fowl in previous reports. In addition, the sporulated oocyst was morphologically different from that of E. grenieri. This suggested that the present strain is a novel species.
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