Effect of gamma-irradiation to the embryos growth of kenaf [Hibiscus cannabinus] and roselle [Hibiscus sabdariffa]
2008
Matsuzaka, T.(Waseda Univ., Tokyo (Japan)) | Okuyama, N. | Fujino, H.
Kenaf and roselle are widely cultivated in many countries and they have been used to produce several products or beverages. Also, these plants are recognized as an environmental friendly crop. The gamma-ray irradiation was commonly used to avoid the spoilage of several vegetables or to inhibit the emergence of larvae from seeds. Recently, radiation breeding was characterized for the creation of new varieties and the improvement of vegetative propagation. As of today, little studies have been reported regarding the radiation sensitivity of these plants. To gain a better understanding on the embryos growth of kenaf and roselle, gamma-ray irradiation was performed on these seeds using 60Co irradiator. A statistically significant difference was observed on germination and embryo growth at irradiated kenaf seeds from control groups. In case of roselle, there was not statistically significant difference in the germination of irradiated seeds from control groups at the same doses. Moreover, the several truncated DNA forms were detected at the irradiated seeds and their embryos in electrophoresis assay. Based on these results, inhibitory effect on germination and embryo growth were closely related to the DNA damages. The present study demonstrates that remarkable difference on the radiation tolerance of seed was found between kenaf and roselle.
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