Suppression of fibroblast cell growth on surfaces coated with conjugates consisting of silk fibroin and chitooligosaccharides
2008
Gotoh, Y.(National Inst. of Agrobiological Sciences, Tsukuba, Ibaraki (Japan)) | Tamada, Y.
Glycoconjugates consisting of silk fibroin (SF) and polycationic chitooligosaccharides (COS) were prepared by the chemical modification of SF with COS using cyanuric chloride (CY) as a coupling spacer. The growth of mouse fibroblast (L-929) cells on glass coverslips coated with the conjugate (COS-CY-SF) was investigated by a cell culture method. The number of cells grown on the COS-CY-SF conjugate-coated surfaces was the lowest compared with surfaces coated with SF and collagen during 7 days of culture, although the cells on the conjugate-coated surfaces were able to proliferate. Moreover, cell spreading on the conjugate coated-surfaces was prevented over 7 days, while the cells on the surfaces coated with SF and collagen exhibited a well-spread morphology within 5 days. These results reveal the cell-resistant properties of the COS-CY-SF conjugated-coated surfaces and the potential use of the conjugate-coatings for implant applications.
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