Bactericidal effect of superfine and high density ozone water on Salmonella Enteritidis
2009
Takagi, M.(Japan. Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Tokyo. National Veterinary Assay Lab.) | Iwata, T. | Akiba, M. | Matsumura, E. | Hagiwara, N. | Kamio, T.
The bactericidal effect of superfine and high density ozone water (nano pico ozone water, ozone water) on Salmonella Enteritidis was evaluated. More than 8 log unit of SE were killed instantaneously in this ozone water. To achieve clear bactericidal effects, a volume of ozone water (10 ppm) of 500 times that of a sample is needed. The minimum effective concentration for 1 mL of SE growth culture sample (about 10E8 cfu) was 4 ppm. This ozone water was stable for a comparatively long time and remained effective after it had remained at room temperature for 30 minutes. This ozone water exhibited bactericidal effects in the presence of organic materials (chicken feces, egg, and chicken meat). A volume of this ozone water (10 ppm) of 3,000 times for 1 mL of a sample with chicken feces and SE growth culture (10E8 cfu) also exhibited bactericidal effects. The effectiveness of this ozone water depended on its temperature. Jet washing of SE-contaminated egg (10E6/egg sample) for one minute sterilized the contaminated egg surface. Jet washing for more than 1.5 minutes with ozone water as same condition against the SE contaminating chicken meat (10E2/meat sample) was able to sterilize the surface of contaminated chicken meat. These results suggest that a low concentration (4 ppm) of this ozone water instantaneously killed bacteria and that ozone water was therefore useful for disinfecting materials in animal houses and slaughter houses and for sterilizing surfaces of chicken eggs or chicken carcasses.
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