Receptor-mediated endocytosis of lipid and lipophorin by the larval fat body, adult ovary and testis in the wax moth Galleria mellonella
2007
Jung, E.S. (Hanseo University, Seosan, Republic of Korea) | Yun, H.K. (Hanseo University, Seosan, Republic of Korea), E-mail: [email protected]
Lipophorin (Lp) in the hemolymph of insects is known to selectively deliver lipids from sites of absorption or synthesis to sites of storage and utilization, such as the fat body, ovary and testis; however, no study regarding this has been reported in Galleria mellonella. In the present study, we examined the process by which Lp is taken up into the larval fat body, adult ovary and adult testis, and the transfer of lipid by Lp to these tissues in Galleria mellonella. To investigate the involvement of a receptor in Lp endocytosis, the larval fat body, adult ovary and adult testis were incubated for 1 h at room temperature with fluorescein isothiocyanate (FITC)-Lp, FITC-Lp plus unlabeled Lp, and FITC-Lp plus suramin, a receptor endocytic inhibitor.
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