Analysis of the inhibitory mechanism of D-allose on MOLT-4F leukemia cell proliferation
2009
Hirata, Y.(Kagawa Univ., Miki (Japan). Faculty of Medicine) | Saito, M. | Tsukamoto, I. | Yamaguchi, F. | Sui, L. | Kamitori, K. | Dong, Y. | Uehara, E. | Konishi, R. | Janjua, N. | Tokuda, M.
D-Allose, the C-3 epimer of D-glucose, is one of the rare sugars found in nature. In the present study, we have elucidated for the first time that various leukemia cell lines have different susceptibility to anti-proliferative activity of D-allose, and that this difference is related to the difference in induction of thioredoxin interacting protein (TXNIP) expression. We examined 5 leukemia cell lines (MOLT-4F, IM-9, HL-60, BALL-1 and Daudi), and found that MOLT-4F (T-cell lymphoblastic leukemia) had the highest susceptibility to D-allose, and that Daudi (Burkitt's lymphoma) had the lowest. D-Allose significantly slowed the cell cycle progression without causing apoptosis of MOLT-4F cells. Intracellular TXNIP expression was specifically and markedly enhanced in MOLT-4F cells by D-allose treatment, and subsequent increase of p27sup(kip1), a cell cycle inhibitor, was observed. On the other hand, D-allose did not increase TXNIP and p27sup(kip1) levels at all in Daudi cells. These results indicate that D-allose suppresses MOLT-4F cell proliferation possibly by the inhibition of cell cycle progression via induction of TNXIP expression.
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