Acanthamoeba infection in cultured oscar Astronotus ocellatus
2009
Laoprasert, T.(Aquatic Animal Health Research Inst., Bangkok (Thailand)) | Yagita, K. | Shimogawara, R. | Chinabut, S. | Hatai, K.
In 2000, an amoebic infection was found in oscar (Astronotus ocellatus), a tropical fish, in Thailand. Amoebae isolated from these oscar were classified into the genus Acanthamoeba. The isolation, morphological identification and biological characteristics of Acanthamoeba isolated from the affected oscar are described. We investigated the growth of isolates cultured on Escherichia coli (E. coli) agar plates under various conditions, and examined the effects of environmental conditions on the growth of trophozoites. Effects of temperature, pH and salinity were examined. The diameters of amoeba colonies in all experimental agar plates were observed and measured daily for 7 days. If amoebae did not grow, survival was confirmed by inoculation onto a new E. coli agar plate at the end of the experiment. As a result, it was demonstrated that Acanthamoeba isolated from the oscar could grow at 5 to 45degC, pH 3-11 and 0-10% NaCl. The results of this study indicated that Acanthamoeba sp. isolated from oscar can grow in a broad range of environmental conditions.
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