Development and evaluation of an immunochromatography kit for detection of white spot virus in shrimp
2009
Fukuda, K.(National Fisheries Univ., Shimonoseki, Yamaguchi (Japan)) | Kondo, M. | Inagawa, H. | Chongthaleong, A. | Becerra, L. | Nishi, K. | Okuyama, A. | Aoki, T. | Takahashi, Y.
Performance of an immunochromatography kit (IC kit) for detection of the white spot virus in cultured shrimp was evaluated. The IC kit's virus detection rate was similar to that of the one-step PCR method, but lower than that of the two-step PCR method. The antibody included in the IC kit recognized the white spot virus (WSV) envelope protein VP28, and the assay had a detection limit of 9.9 x 10E3 viral DNA copies/micro l. Compared to detection based on gross and histopathological examination, this assay could detect virus even from shrimps without apparent disease signs and also few virus positive cells in the stomach and gills. When infected cultured shrimp from various countries and several other infected crustaceans were tested using the IC kit as well as one-step PCR, the IC kit was proven as a more rapid diagnostic tool.
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