Skin Whitening and Skin Immune Activities of the Peptides Isolated from Crassostrea gigas by Ultrasonification Processes
2008
Han, J.G. (Kangwon National University, Chuncheon, Republic of Korea) | Kim, H.S. (Kangwon National University, Chuncheon, Republic of Korea) | Kwon, M.C. (Kangwon National University, Chuncheon, Republic of Korea) | Kim, J.C. (Kangwon National University, Chuncheon, Republic of Korea) | Hwang, B.Y. (SKY007 Co., Gangneung, Republic of Korea) | Lee, H.Y. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: [email protected]
In this study, peptides were isolated from Crassostrea gigas using an ultrasonification process at 40℃. The yield of the peptides was greater than 34%, and their cytotoxicity was found to be less than 22.8% against several cell lines that were treated with the extracts at a dose of 1.0 mg/mL. In addition, the tyrosinase inhibitory and melanin synthesis of the peptides isolated from Crassostrea gigas were also evaluated to determine if they could be used as a potential cosmetic agent. The peptides were found to significantly inhibit the melanin synthesis of the clone M-3 cell line by up to 62.7%. The inhibitory activities of the tyrosinase were observed 34.51% in ascorbic acid, 42.49% in extract with the ultrasonification at 40℃ and 35.37% in 40℃ extract at 1.0 mg/mL concentration, respectively. Finally, when samples were treated with the peptide extracts at a concentration of 0.6 mg/mL, PGE2 expression was significantly decreased. Taken together, these results indicate that Crassostrea gigas may be a source of cosmetic agents capable of improving physiological hyperpigmenting and immuno-modulating skin disorders.
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